goat anti rat timp 1  (R&D Systems)

Journal: Neurobiology of disease

Article Title: TIMP-1 associates with myelin membrane and preserves myelin in injured peripheral nerve.

doi: 10.1016/j.nbd.2025.106892

Figure Lengend Snippet: Fig. 1. Early-phase elevation of TIMP-1 in CCI nerve. A, TIMP-1 (28 kD) immunoblotting in rat sciatic nerve total lysates at days 0 (contralateral), 1, 7, and 28 post- CCI, using GAPDH (36 kD) as a loading control. B, The mean TIMP-1 to GAPDH ratio ± SEM of n = 6 (3 male, 3 female) rats/group of A.; by one-way ANOVA with Tukey’s post-hoc test, or Student’s t-test. C, The mean TIMP-1 to GAPDH ratio ± SEM of n = 3 male vs. 3 female rats/group of B.; by one-way ANOVA with Tukey’s post-hoc test. B-C: n.s, not significant.

Article Snippet: Nonspecific binding was blocked with 10 % normal donkey serum (Sigma, 1 h, room temperature) and incubated overnight with goat anti-rat Timp1 (1:50; Cat# AF580, R&D Systems) and rabbit anti-MBP (1:100; Cat# ab40390, Abcam) primary antibodies (4 ◦C, 16–18 h).

Techniques: Western Blot, Control

Journal: Neurobiology of disease

Article Title: TIMP-1 associates with myelin membrane and preserves myelin in injured peripheral nerve.

doi: 10.1016/j.nbd.2025.106892

Figure Lengend Snippet: Fig. 2. TIMP-1 in myelinating (m)SC and myelin sheath in CCI nerve. A, A myelinated axon (Ax) and mSC unit (a schematic). B, TIMP-1 immunofluores cence in rat sciatic nerve at days 0 (contralateral), 1, and 7 post-CCI; median (interquartile range) immunoreactivity count per (81,380 μm2 endoneurial area) of n = 6–7/group (3–4 male, 3–4 female), 3 sections/n; Kruskal-Wallis test with Dunn-Bonferoni post-hoc were used for p = value Red circle, female; blue square, male. C, MBP (green) and TIMP-1 (red) dual-IF shows TIMP-1 in MBP- reactive mSC cytoplasm (arrowheads) and myelin sheaths (arrows) in CCI nerves; DAPI (blue). Scale bar, 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Nonspecific binding was blocked with 10 % normal donkey serum (Sigma, 1 h, room temperature) and incubated overnight with goat anti-rat Timp1 (1:50; Cat# AF580, R&D Systems) and rabbit anti-MBP (1:100; Cat# ab40390, Abcam) primary antibodies (4 ◦C, 16–18 h).

Techniques:

Journal: Neurobiology of disease

Article Title: TIMP-1 associates with myelin membrane and preserves myelin in injured peripheral nerve.

doi: 10.1016/j.nbd.2025.106892

Figure Lengend Snippet: Fig. 3. Myelin/membrane (mm)TIMP-1 in fractionated CCI nerve. A, FLOT-1 (47 kD, lipid raft marker) and MBP (14, 18 kD, myelin marker) immunoblot ting in rat sciatic nerve sucrose fractions (1−12) at day 1 post-CCI. Rep. of n = 6 (3 male, 3 female) per group, female shown. FLOT-1 and MBP in light/buoyant fractions (4,5,6), defined as myelin/membrane lipid rafts. FLOT-1 in heavy/ non-buoyant fractions (10,11,12). B, TIMP-1 (28 kD) immunoblotting of frac tions (4,5,6) in rat sciatic nerve at day 1 post-CCI. Rep. of n = 6 (3 male, 3 female) per group. TL, total lysate control; M, molecular weight standard. C, LC- MS/MS analysis of rat female sciatic nerve sample [4–6] at day 1 post-CCI, as in A-B, concentrated using an Ultracel-10 membrane filter. Note TIMP-1 (rat, Accession #P30120) and myelin proteins, P0 (rat, MYP0, Accession #P06907) and MBP (rat, Accession #P02688), in the myelin/membrane lipid raft fraction.

Article Snippet: Nonspecific binding was blocked with 10 % normal donkey serum (Sigma, 1 h, room temperature) and incubated overnight with goat anti-rat Timp1 (1:50; Cat# AF580, R&D Systems) and rabbit anti-MBP (1:100; Cat# ab40390, Abcam) primary antibodies (4 ◦C, 16–18 h).

Techniques: Membrane, Marker, Western Blot, Control, Molecular Weight, Liquid Chromatography with Mass Spectroscopy

Journal: Neurobiology of disease

Article Title: TIMP-1 associates with myelin membrane and preserves myelin in injured peripheral nerve.

doi: 10.1016/j.nbd.2025.106892

Figure Lengend Snippet: Fig. 4. Time-course of (mm)TIMP-1 in CCI nerve. A, TIMP-1 (28 kD) immunoblotting in fractionated rat sciatic nerve in samples [4–6] and [10–12] at days 0 (contralateral) and 1 post-CCI. FLOT-1 (47 kD) as lipid rafts marker/loading control. Exposure times: 1-12 min. B, The mean TIMP-1 to FLOT-1 ratio in sample [4–6] at days 0, 1, 7, and 28 post-CCI ± SEM of n = 6–8/group (2–4 male, 3–5 female); by one-way ANOVA and Student’s t-test. -Representative of A.

Article Snippet: Nonspecific binding was blocked with 10 % normal donkey serum (Sigma, 1 h, room temperature) and incubated overnight with goat anti-rat Timp1 (1:50; Cat# AF580, R&D Systems) and rabbit anti-MBP (1:100; Cat# ab40390, Abcam) primary antibodies (4 ◦C, 16–18 h).

Techniques: Western Blot, Marker, Control

Journal: Journal of Immunology Research

Article Title: Achalasia—An Autoimmune Inflammatory Disease: A Cross-Sectional Study

doi: 10.1155/2015/729217

Figure Lengend Snippet: Proteins involved in extracellular matrix turnover in achalasia. (a) Representative photomicrograph from a control and an idiopathic achalasia tissue in which an abundant inflammatory infiltrate is observed. Original magnification was ×200. (b) Histological findings in biopsies from achalasia patients. (c) Immunoreactive cells. Original magnification was ×320. (d) Results are expressed as mean (yellow line), median (black line), and 5th/95th percentiles of MMP-9 + cells. (e) Immunohistochemistry for TIMP-1 in control and achalasia sections. Arrows depict immunoreactive cells. Original magnification was ×320. (f) Results are expressed as mean (black line), median (yellow line), and 5th/95th percentiles of TIMP-1 + cells.

Article Snippet: Tissues were incubated for 18 h at 4°C with goat polyclonal anti-human MMP-9, TIMP-1, TGF- β 1, IL-22, IL-17A, FAS, or mouse monoclonal IgG 1 HSV-1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 10 μ g/mL.

Techniques: Immunohistochemistry

Journal: Journal of Immunology Research

Article Title: Achalasia—An Autoimmune Inflammatory Disease: A Cross-Sectional Study

doi: 10.1155/2015/729217

Figure Lengend Snippet: Percentage of immunoreactive cells in achalasia patients.

Article Snippet: Tissues were incubated for 18 h at 4°C with goat polyclonal anti-human MMP-9, TIMP-1, TGF- β 1, IL-22, IL-17A, FAS, or mouse monoclonal IgG 1 HSV-1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 10 μ g/mL.

Techniques: